Recombinant Rat Stromal-Cell Derived Factor-1 alpha/CXCL12α

Cat No.

RP00074

Product Specifications

Product Type

Recombinant protein

Applications

Bioactivity, Endotoxin

Source

E. coli.

UniProt No.Q9QZD1
Purity

> 97 % by SDS-PAGE and HPLC analyses.

Bioactivity

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 50-100 ng/ml.

Endotoxin

Less than 1 EU/μg of rRtSDF-1α/CXCL12α as determined by LAL method.

Molecular Weight

Approximately 7.9 kDa, a single non-glycosylated polypeptide chain containing 68 amino acids.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.

Alternative Names

SDF-1α/CXCL12α, Sdf1, Rat

Storage

-20C

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 C. Further dilutions should be made in appropriate buffered solutions.

Research Area

Immunology