Recombinant Rat Interleukin-6

Cat No.

RP00101

Product Specifications

Product Type

Recombinant protein

Applications

Bioactivity, Endotoxin

Source

E. coli.

UniProt No.P20607
Purity

> 95 % by SDS-PAGE and HPLC analyses.

Bioactivity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using IL-6-dependent Mouse 7TD1 cells is less than 0.01 ng/ml, corresponding to a specific activity of > 1.0 × 108 IU/mg.

Endotoxin

Less than 1 EU/μg of rRtIL-6 as determined by LAL method.

Molecular Weight

Approximately 21.7 kDa, a single, non-glycosylated polypeptide chain containing 187 amino acids

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

Alternative Names

IL-6, ILg6, Ifnb2, IL6, Rat

Storage

-20C

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 10mM HCl to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Research Area

Immunology