Recombinant Rat Fibroblast Growth Factor-21
RP00126
Product Specifications
| Product Type | Recombinant protein |
|---|---|
| Applications | Bioactivity, Endotoxin |
| Source | E. coli. |
| UniProt No. | Q8VI80 |
| Purity | > 95 % by SDS-PAGE and HPLC analyses. |
| Bioactivity | Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 μg/ml, corresponding to a specific activity of > 2.0 × 103 IU/mg in the presence of 5 μg/ml of rMuKlotho-β and 10 μg/ml of heparin. |
| Endotoxin | Less than 1EU/μg of rRtFGF-21 as determined by LAL method. |
| Molecular Weight | Approximately 19.7 kDa, a single non-glycosylated polypeptide chain containing 180 amino acids. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, 500 mM NaCl, pH 7.4. |
| Alternative Names | FGF-21, Fgf8c, FGF21, Rat |
| Storage | -20C |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Research Area | Immunology |