Recombinant Rat Eotaxin/CCL11

Cat No.

RP00129

Product Specifications

Product Type

Recombinant protein

Applications

Bioactivity, Endotoxin

Source

E. coli.

UniProt No.P97545
Purity

> 96 % by SDS-PAGE and HPLC analyses.

Bioactivity

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using purified eosinophils is in a concentration range of 0.1-1.0 μg/ml.

Endotoxin

Less than 1 EU/μg of rRtEotaxin/CCL11 as determined by LAL method.

Molecular Weight

Approximately 8.4 kDa, a single non-glycosylated polypeptide chain containing 74 amino acids.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.

Alternative Names

Eotaxin/CCL11, Scya11, Rat

Storage

-20C

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 C. Further dilutions should be made in appropriate buffered solutions.

Research Area

Immunology