Recombinant Rat Cerebral Dopamine Neurotrophic Factor

Cat No.

RP00137

Product Specifications

Product Type

Recombinant protein

Applications

Bioactivity, Endotoxin

Source

E. coli.

UniProt No.P0C5I0
Purity

> 97 % by SDS-PAGE and HPLC analyses.

Bioactivity

Fully biologically active when compared to standard. It is able to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons when immobilized at 5 – 25 μg/mL on a nitrocellulose-coated microplate.

Endotoxin

Less than 0.1 EU/μg of rRtCDNF as determined by LAL method.

Molecular Weight

Approximately 18.8 kDa, a single non-glycosylated polypeptide chain containing 163 amino acids.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

Alternative Names

CDNF, Armetl1, Rat

Storage

-20C

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Research Area

Immunology