Recombinant Mouse Macrophage Inflammatory Protein-1 alpha/CCL3

Cat No.

RP00298

Product Specifications

Product Type

Recombinant protein

Applications

Bioactivity, Endotoxin

Source

E. coli.

UniProt No.P10855
Purity

> 98 % by SDS-PAGE and HPLC analyses.

Bioactivity

Fully biologically active when compared to standard. The biological activity determined by a chemoattract bioassay using Mouse splenocytes is in a concentration range of 10-100 ng/ml.

Endotoxin

Less than 0.1 EU/μg of rMuMIP-1α/CCL3 as determined by LAL method.

Molecular Weight

Approximately 7.9 kDa protein containing 69 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in 30 % Acetonitrile and 0.1 % TFA.

Alternative Names

MIP-1α/CCL3, AI323804, CCL, G0S19-, G0S19-1, LD78, LD78alpha, MIP-, MIP-1alpha, MIP1, MIP1-, MIP1-(a), MIP1-alpha, Mi, Mip1a, Scy, Scya3, Mouse

Storage

-20C

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 C. Further dilutions should be made in appropriate buffered solutions.

Research Area

Others