Recombinant Mouse Midkine
RP00301
Product Specifications
| Product Type | Recombinant protein |
|---|---|
| Applications | Bioactivity, Endotoxin |
| Source | E. coli. |
| UniProt No. | P12025 |
| Purity | > 96 % by SDS-PAGE and HPLC analyses. |
| Bioactivity | Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration range of 10-100 ng/ml. |
| Endotoxin | Less than 1 EU/μg of rMuMidkine as determined by LAL method. |
| Molecular Weight | Approximately 13.2 kDa, a single non-glycosylated polypeptide chain containing 120 amino acids. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH7.4. |
| Alternative Names | MDK, MK, Mek, Midkine, Mouse |
| Storage | -20C |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 C. Further dilutions should be made in appropriate buffered solutions. |
| Research Area | Immunology |