Recombinant Mouse Interferon-lambda 2/Interleukin-28A

Cat No.

RP00408

Product Specifications

Product Type

Recombinant protein

Applications

Bioactivity, Endotoxin

Source

E. coli.

UniProt No.Q4VK74;B9EK98
Purity

> 95 % by SDS-PAGE and HPLC analyses.

Bioactivity

Fully biologically active when compared to standard. The ED50 as determined by an anti-viral assay using human HepG2 cells infected with encephalomyocarditis is less than 3 ng/ml, corresponding to a specific activity of > 3.3 × 105 IU/mg.

Endotoxin

Less than 0.1 EU/μg of rMuIFN-λ2/IL-28A as determined by LAL method.

Molecular Weight

Approximately 19.7 kDa, a single non-glycosylated polypeptide chain containing 174 amino acids.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4, with 5 % trehalose.

Alternative Names

IFN-λ2/IL-28A, Il; Il28a; IL-28A; If1ia1; EG330496, Ifnl2, Mouse

Storage

-20C

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Research Area

Immunology