Recombinant Mouse Fibroblast Growth Factor-8
RP00453
Product Specifications
| Product Type | Recombinant protein |
|---|---|
| Applications | Bioactivity, Endotoxin |
| Source | E. coli. |
| UniProt No. | P37237 |
| Purity | > 97 % by SDS-PAGE and HPLC analyses. |
| Bioactivity | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using Mouse balb/c 3T3 cells is less than 5.0 ng/ml, corresponding to a specific activity of > 2.0 × 105 IU/mg in the presence of 10 μg/ml of heparin. |
| Endotoxin | Less than 1EU/μg of rMuFGF-8 as determined by LAL method. |
| Molecular Weight | Approximately 28.1 kDa, a single non-glycosylated polypeptide chain containing 246 amino acids. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4, 500 mM NaCl. |
| Alternative Names | FGF-8, Ai, Aigf, Fgf8, Fgf6c, Mouse |
| Storage | -20C |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Research Area | Immunology |