Recombinant Mouse Keratinocyte Growth Factor-1/FGF-7

Cat No.

RP00454

Product Specifications

Product Type

Recombinant protein

Applications

Bioactivity, Endotoxin

Source

E. coli.

UniProt No.P36363
Purity

> 96 % by SDS-PAGE and HPLC analyses.

Bioactivity

Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 10 ng/ml, corresponding to a specific activity of > 1.0 × 105 IU/mg.

Endotoxin

Less than 1 EU/μg of rMuKGF-1/FGF-7 as determined by LAL method.

Molecular Weight

Approximately 18.7 kDa, a single, non-glycosylated polypeptide chain containing 163 amino acids.

Formulation

Lyophilized from a 0.2 μm filtered solution in 20 mM PB, pH 8.0, 1 M NaCl.

Alternative Names

FGF-7/KGF-1, Kgf; Fgf5b, FGF7, Mouse

Storage

-20C

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Research Area

Immunology