Recombinant Mouse Desert Hedgehog Cys23IleIle
RP00485
Product Specifications
| Product Type | Recombinant protein |
|---|---|
| Applications | Bioactivity, Endotoxin |
| Source | E. coli. |
| UniProt No. | Q61488 |
| Purity | > 98 % by SDS-PAGE and HPLC analyses. |
| Bioactivity | Fully biologically active when compared to standard. The ED50 as determined by its ability to induce alkaline phosphatase production by Mouse MC3T3-E1 cells is 5-20 μg/ml. |
| Endotoxin | Less than 0.1 EU/μg of rMuDHH C23II as determined by LAL method. |
| Molecular Weight | Approximately 20.0 kDa, a single non-glycosylated polypeptide chain containing 177 amino acids. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4, 1 mM DTT, 0.05 % Tween-80. |
| Alternative Names | DHH C23II, C78960, Mouse |
| Storage | -20C |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 C. Further dilutions should be made in appropriate buffered solutions. |
| Research Area | Others |