Recombinant Human TP53-induced Glycolysis and Apoptosis Regulator-TAT
RP00821
Product Specifications
| Product Type | Recombinant protein |
|---|---|
| Applications | Bioactivity, Endotoxin |
| Source | E. coli. |
| UniProt No. | Q9NQ88 |
| Purity | > 96 % by SDS-PAGE and HPLC analyses. |
| Bioactivity | Fully biologically active when compared to standard. The biological activity determined by its ability to protect U2OS cells from apoptosis induced by hydrogen peroxide is in a concentration range of 0.1-5.0 μg/ml, after pretreating with rHuTIGAR-TAT for 4 hours. |
| Endotoxin | Less than 0.1 EU/μg of rHuTIGAR-TAT as determined by LAL method. |
| Molecular Weight | Approximately 31.7 kDa, a single non-glycosylated polypeptide chain containing 283 amino acids. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.1, 350 mM NaCl, 10 % Trehalose. |
| Alternative Names | TIGAR, C12orf5, FR2BP, Human |
| Storage | -20C |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 C. Further dilutions should be made in appropriate buffered solutions. |
| Research Area | Others |