Recombinant Human Neuregulin 1-beta2 EGF-like domain

Cat No.

RP01197

Product Specifications

Product Type

Recombinant protein

Applications

Bioactivity, Endotoxin

Source

E. coli.

UniProt No.Q02297
Purity

> 96 % by SDS-PAGE and HPLC analyses.

Bioactivity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using serum free human MCF-7 cells is less than 50 ng/ml, corresponding to a specific activity of > 2.0 × 104 IU/mg.

Endotoxin

Less than 1 EU/μg of rHuNRG1-β2 as determined by LAL method.

Molecular Weight

Approximately 7.0 kDa, a single non-glycosylated polypeptide chain containing 61 amino acids.

Formulation

Lyophilized from a 0.2 μm filtered solution in 20 mM PB, pH 7.0, containing 0.5 % HAS and 2 % mannitol.

Alternative Names

NRG1-β2, Human

Storage

-20C

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Research Area

Immunology