Recombinant Human Macrophage Inflammatory Protein-1 alpha/CCL3

Cat No.

RP01282

Product Specifications

Product Type

Recombinant protein

Applications

Bioactivity, Endotoxin

Source

E. coli.

UniProt No.P10147
Purity

> 96 % by SDS-PAGE and HPLC analyses.

Bioactivity

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 1.0-10 ng/ml.

Endotoxin

Less than 1 EU/ug of rHuMIP-1α/CCL3 as determined by LAL method.

Molecular Weight

Approximately 7.8 kDa protein containing 70 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 100 mM NaCl.

Alternative Names

MIP-1α/CCL3, G0S19-1, LD78ALPHA, MIP-1-alpha, MIP1A, SCYA3, Human

Storage

-20C

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 C. Further dilutions should be made in appropriate buffered solutions.

Research Area

Immunology