Recombinant Human Interleukin-36 alpha, 153a.a.
RP01503
Product Specifications
| Product Type | Recombinant protein |
|---|---|
| Applications | Bioactivity, Endotoxin |
| Source | E. coli. |
| UniProt No. | Q9UHA7 |
| Purity | > 95 % by SDS-PAGE and HPLC analyses. |
| Bioactivity | Fully biologically active when compared to standard. The ED50 as determined by inducing IL-8 secretion in human preadipocytes is less than 10 ng/ml, corresponding to a specific activity of > 1.0 × 105 IU/mg. |
| Endotoxin | Less than 1 EU/μg of rHuIL-36α, 153a.a. as determined by LAL method. |
| Molecular Weight | Approximately 17.1 kDa, a single non-glycosylated polypeptide chain containing 153 amino acids. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM Tris, 300 mM NaCl, pH 8.0, 0.1 % Tween 80. |
| Alternative Names | IL-36α, IL36A, FIL1, FIL1(EPSILON), FIL1E, IL-1F6, IL1(EPSILON), IL1F6, Human |
| Storage | -20C |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 C. Further dilutions should be made in appropriate buffered solutions. |
| Research Area | Immunology |