Recombinant Human Fibroblast Growth Factor-23
RP01801
Product Specifications
| Product Type | Recombinant protein |
|---|---|
| Applications | Bioactivity, Endotoxin |
| Source | E. coli. |
| UniProt No. | Q9GZV9 |
| Purity | > 95 % by SDS-PAGE and HPLC analyses. |
| Bioactivity | Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 μg/ml, corresponding to a specific activity of > 2.0 × 103 IU/mg in the presence of 0.3 μg/ml of rMuKlotho and 10 μg/ml of heparin. |
| Endotoxin | Less than 1 EU/μg of rHuFGF-23 as determined by LAL method. |
| Molecular Weight | Approximately 25.3 kDa, a single non-glycosylated polypeptide chain containing 227 amino acids. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
| Alternative Names | FGF-23, FGF23, ADHR, FGFN, HFTC2, HPDR2, HYPF, PHPTC, Human |
| Storage | -20C |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Research Area | Immunology |