Recombinant protein

Bioactivity, Endotoxin

E. coli.

> 95 % by SDS-PAGE and HPLC analyses.

Assay #1: Fully biologically active when compared to standard. The biological activity is measured by its binding ability in a functional ELISA. Immobilized rHuFGF R4 at 5 μg/ml can bind rHuFGF-19 with a linear range of 3-200 ng/ml.
Assay #2: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using Mouse Balb/c 3T3 cells is less than 150 ng/ml, corresponding to a specific activity of > 6.7 × 103 IU/mg.

Less than 1 EU/μg of rHuFGF-19 as determined by LAL method.

Approximately 21.8 kDa, a single non-glycosylated polypeptide chain containing 195 amino acids.

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

FGF-19, FGF19, Human

-20C

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Immunology