Recombinant Human Cysteine-rich Angiogenic Inducer 61
RP01946
Product Specifications
| Product Type | Recombinant protein |
|---|---|
| Applications | Bioactivity, Endotoxin |
| Source | E. coli. |
| UniProt No. | O00622; Q6FI18; O95694;Q9UID7 |
| Purity | > 95 % by SDS-PAGE and HPLC analyses. |
| Bioactivity | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using Mouse Balb/3T3 cells is less than 3.0 μg/ml, corresponding to a specific activity of > 330 IU/mg. |
| Endotoxin | Less than 1 EU/μg of rHuCYR61 as determined by LAL method. |
| Molecular Weight | Approximately 39.4 kDa, a single non-glycosylated polypeptide chain containing 357 amino acids. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in citrate buffer solution, 300 mM NaCl, pH 3.0. |
| Alternative Names | CYR61, CCN1, GIG1, IGFBP10, Human |
| Storage | -20C |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Research Area | Immunology |