Recombinant Human Bone Morphogenetic Protein 2

Cat No.

RP02240

Product Specifications

Product Type

Recombinant protein

Applications

Bioactivity, Endotoxin

Source

E. coli.

UniProt No.P12643
Purity

> 95 % by SDS-PAGE and HPLC analyses.

Bioactivity

Fully biologically active when compared to standard. The ED50 as determined by inducing alkaline phosphatase production of Mouse ATDC5 cells is less than 200 ng/ml, corresponding to a specific activity of > 5.0 × 103 IU/mg.

Endotoxin

Less than 1 EU/μg of rHuBMP-2 as determined by LAL method.

Molecular Weight

Approximately 26.0 kDa, a homodimeric protein consisting of two 115 amino acid non-glycosylated polypeptide chains.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution containing 10 mM sodium citrate pH 3.5.

Alternative Names

BMP-2, BDA2A, SSFSC, SSFSC1, BMP2, Human

Storage

-20C

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled H2O to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. Do not reconstitute in PBS or cell culture media.

Research Area

Immunology