Recombinant Human Beta-defensin 3

Cat No.

RP02268

Product Specifications

Product Type

Recombinant protein

Applications

Bioactivity, Endotoxin

Source

E. coli.

UniProt No.P81534
Purity

> 98 % by SDS-PAGE and HPLC analyses.

Bioactivity

Fully biologically active when compared to standard. The ED50 as determined by anti-microbial activity against E.coli. is less than 30 μg/ml, corresponding to a specific activity of > 33.3 IU/mg.

Endotoxin

Less than 1 EU/μg of rHuBD-3 as determined by LAL method.

Molecular Weight

Approximately 5.2 kDa, a single non-glycosylated polypeptide chain containing 45 amino acids.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 130 mM NaCl.

Alternative Names

BD-3, BD3, DEFB103B, DEFB-3, DEFB103, DEFB3, HBD-3, HBD3, HBP-3, HBP3 Human

Storage

-20C

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Research Area

Immunology