Recombinant Human B Cell Activating Factor

Cat No.

RP02285

Product Specifications

Product Type

Recombinant protein

Applications

Bioactivity, Endotoxin

Source

E. coli.

UniProt No.Q9Y275
Purity

> 95 % by SDS-PAGE and HPLC analyses.

Bioactivity

Fully biologically active when compared to standard. The ED50 as determined by a mouse splenocyte survival assay is 0.5-2 μg/ml.

Endotoxin

Less than 1 EU/μg of rHuBAFF as determined by LAL method.

Molecular Weight

Approximately 17.2 kDa, a single non-glycosylated polypeptide chain containing 153 amino acids.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.0.

Alternative Names

TNFSF13B, BAFF, BLYS, CD257, DTL, TALL-1, TALL1, THANK, TNFSF20, TNLG7A, ZTNF4, Human

Storage

-20C

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Research Area

Immunology