Recombinant Hirudin
RP02410
Product Specifications
| Product Type | Recombinant protein |
|---|---|
| Applications | Bioactivity, Endotoxin |
| Source | Pichia Pastoris |
| UniProt No. | P01050 |
| Purity | > 96 % by SDS-PAGE and HPLC analyses. |
| Bioactivity | The biological activity is determined by chromogenic assay, 1 unit is defined as the amount of Hirudin that neutralizes 1 unit of the WHO preparation 89/588 of thrombin. The specific activity is no less than 14, 000 ATU/mg protein. |
| Endotoxin | Less than 1 EU/μg of rHirudin as determined by LAL method. |
| Molecular Weight | Approximately 6.7 kDa, a single non-glycosylated polypeptide chain containing 63 amino acid residues. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of 20 mM PBS, pH 7.0, containing 2 % mannitol. |
| Alternative Names | Hirudin, Recombinant |
| Storage | -20C |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Research Area | Cardiovascular |