Recombinant Human Fms-related Tyrosine Kinase 3 Ligand

Cat No.

RP01772

Product Specifications

Product Type

Recombinant protein

Applications

Bioactivity, Endotoxin

Source

E. coli.

UniProt No.P36888
Purity

> 97 % by SDS-PAGE and HPLC analyses.

Bioactivity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human AML5 cells is less than 1.0 ng/ml, corresponding to a specific activity of > 1.0 × 106 IU/mg.

Endotoxin

Less than 1 EU/μg of rHuFlt3-Ligand as determined by LAL method.

Molecular Weight

Approximately 17.6 kDa, a single non-glycosylated polypeptide chain containing 155 amino acids.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.0.

Alternative Names

Flt-3 Ligand, CD135, FLK-2, FLK2, STK1, Human

Storage

-20C

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Research Area

Immunology