Recombinant Human Indian Hedgehog Cys28IleIle
RP01573
Product Specifications
| Product Type | Recombinant protein |
|---|---|
| Applications | Bioactivity, Endotoxin |
| Source | E. coli. |
| UniProt No. | Q14623 |
| Purity | > 96 % by SDS-PAGE and HPLC analyses. |
| Bioactivity | Fully biologically active when compared to standard. The ED50 as determined by its ability to induce alkaline phosphatase production by C3H10T1/2(CCL-226) cells is 3.0-10 μg/ml. |
| Endotoxin | Less than 1 EU/μg of rHuIHH C28II as determined by LAL method. |
| Molecular Weight | Approximately 19.8 kDa, a single non-glycosylated polypeptide chain containing 176 amino acids. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 1 × PBS, pH 7.4. |
| Alternative Names | IHH C28II, BDA1, HHG2, Human |
| Storage | -20C |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Research Area | Others |