Recombinant Human Interleukin-2

Cat No.

RP01528

Product Specifications

Product Type

Recombinant protein

Applications

Bioactivity, Endotoxin

Source

E. coli.

UniProt No.P60568
Purity

> 97 % by SDS-PAGE and HPLC analyses.

Bioactivity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using Mouse CTLL-2 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 × 107 IU/mg.

Endotoxin

Less than 1.0 EU/μg of rHuIL-2 as determined by LAL method.

Molecular Weight

Approximately 15.4 kDa, a single non-glycosylated polypeptide chain containing 133 amino acids.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 5.0.

Alternative Names

IL-2, IL2, TCGF, lymphokine, Human

Storage

-20C

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled H2O to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. Do not reconstitute in cell culture media directly.

Research Area

Immunology