Recombinant Human Interleukin-36 alpha, 158a.a.

Cat No.

RP01504

Product Specifications

Product Type

Recombinant protein

Applications

Bioactivity, Endotoxin

Source

E. coli.

UniProt No.Q9UHA7
Purity

> 95 % by SDS-PAGE and HPLC analyses.

Bioactivity

Fully biologically active when compared to standard. The specific activity determined by its ability in a functional ELISA. Immobilized rHuIL-36α at 1 μg/mL can bind recombinant human IL-1 Rrp2 Fc Chimera with a range of 0.15-5 μg/mL.

Endotoxin

Less than 1 EU/μg of rHuIL-36α, 158a.a. as determined by LAL method.

Molecular Weight

Approximately 17.7 kDa, a single non-glycosylated polypeptide chain containing 158 amino acids.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH 7.4.

Alternative Names

IL-36α, IL36A, FIL1, FIL1(EPSILON), FIL1E, IL-1F6, IL1(EPSILON), IL1F6, Human

Storage

-20C

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 C. Further dilutions should be made in appropriate buffered solutions.

Research Area

Immunology