Recombinant Human LR3 Insulin-like Growth Factor-1
RP01368
Product Specifications
| Product Type | Recombinant protein |
|---|---|
| Applications | Bioactivity, Endotoxin |
| Source | E. coli. |
| UniProt No. | P05019 |
| Purity | >98 % by SDS-PAGE analyses.>90 % by RP-HPLC analyses. |
| Bioactivity | Assay 1: Fully biologically active when compared to standard. Measured in a serum-free cell proliferation assay using human MCF-7 cells. The ED50 for this effect is 0.3-1.5 ng/ml, corresponding to a specific activity of > 6.7 × 105 IU/mg.Assay 2: Fully biologically active when compared to standard. The ED50 as determined by the stimulation of protein synthesis using rat L6 myoblasts is less than 10 ng/ml, corresponding to a specific activity of > 1.0 × 105 IU/mg. |
| Endotoxin | Less than 0.01 EU/μg of rHuLR3 IGF-1 as determined by LAL method. |
| Molecular Weight | Approximately 9.1 kDa, a single non-glycosylated polypeptide chain containing 83 amino acids. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.2. |
| Alternative Names | LR3 IGF-1, IGF, IGF-I, IGFI, MGF, Human |
| Storage | -20C |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Research Area | Immunology |