Recombinant Human Osteoprotegerin
RP01180
Product Specifications
| Product Type | Recombinant protein |
|---|---|
| Applications | Bioactivity, Endotoxin |
| Source | E. coli. |
| UniProt No. | O00300 |
| Purity | > 95 % by SDS-PAGE and HPLC analyses. |
| Bioactivity | Fully biologically active when compared to standard. The ED50 as determined by neutralizing the stimulation of U937 cells is less than 10 ng/ml, corresponding to a specific activity of > 1.0 × 105 IU/mg in the presence of 10 ng/mL soluble rHuRANKL (sRANKL). |
| Endotoxin | Less than 1 EU/ug of rHuOPG as determined by LAL method. |
| Molecular Weight | Approximately 19.7 kDa, a single non-glycosylated polypeptide chain containing 173 amino acids. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, 150 mM NaCl, pH 6.0. |
| Alternative Names | TNFRSF11B, OCIF, OPG, PDB5, TR1, Human |
| Storage | -20C |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 C. Further dilutions should be made in appropriate buffered solutions. |
| Research Area | Immunology |