Recombinant Human soluble Receptor Activator of Nuclear Factor kappa-B Receptor
RP00883
Product Specifications
| Product Type | Recombinant protein |
|---|---|
| Applications | Bioactivity, Endotoxin |
| Source | E. coli. |
| UniProt No. | Q9Y6Q6 |
| Purity | > 98 % by SDS-PAGE and HPLC analyses. |
| Bioactivity | Fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit sRANK Ligand induced nuclear factor kappa B(NFkappaB) in RAW 264.7 cells is less than 50 ng/ml, corresponding to a specific activity of > 2.0 × 104 IU/mg in the presence of 15 ng/ml of recombinant sRANK Ligand. |
| Endotoxin | Less than 0.1 EU/μg of rHusRANK Receptor as determined by LAL method. |
| Molecular Weight | Approximately 19.1 kDa, a single non-glycosylated polypeptide chain containing 174 amino acids. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM Tris-HCl, pH 8.0, 150mM NaCl. |
| Alternative Names | sRANK Receptor, Human |
| Storage | -20C |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Research Area | Immunology |