Recombinant Human Stromal-Cell Derived Factor-1 alpha/CXCL12α

Cat No.

RP00953

Product Specifications

Product Type

Recombinant protein

Applications

Bioactivity, Endotoxin

Source

E. coli.

UniProt No.P48061
Purity

> 97 % by SDS-PAGE and HPLC analyses.

Bioactivity

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using PHA and rHuIL-2 activated human peripheral blood T-lymphocytes is in a concentration range of 20-80 ng/ml.

Endotoxin

Less than 1 EU/μg of rHuSDF-1α/CXCL12α as determined by LAL method.

Molecular Weight

Approximately 8.0 kDa, a single non-glycosylated polypeptide chain containing 68 amino acids.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB pH 7.0, 130 mM NaCl.

Alternative Names

SDF-1α/CXCL12α, IRH, PBSF, SCYB12, SDF1, TLSF, TPAR1,Human

Storage

-20C

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Research Area

Immunology