Recombinant Human Ubiquitin Conjugating Enzyme E2 D3, His
RP00728
Product Specifications
| Product Type | Recombinant protein |
|---|---|
| Applications | Bioactivity, Endotoxin |
| Source | E. coli. |
| UniProt No. | P61077 |
| Purity | > 95 % by SDS-PAGE and HPLC analyses. |
| Bioactivity | Data is not available. |
| Endotoxin | Less than 1 EU/μg of rHuUBE2D3/UBC5C, His as determined by LAL method. |
| Molecular Weight | Approximately 17.7 kDa, a single non-glycosylated polypeptide chain containing 147 amino acids (a.a.) of human UBE2D3/UBC5C and 8 a.a. vector sequence including 6 × His tag at N-terminus. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, 1mM DTT, pH 7.5. |
| Alternative Names | UBE2D3, E2(17)KB3, UBC4/5, UBCH5C, Human |
| Storage | -20C |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Research Area | Others |