Recombinant Human Ubiquitin Conjugating Enzyme E2 L3, His

Cat No.

RP00725

Product Specifications

Product Type

Recombinant protein

Applications

Bioactivity, Endotoxin

Source

E. coli.

UniProt No.P68036
Purity

> 95 % by SDS-PAGE and HPLC analyses.

Bioactivity

Data is not available.

Endotoxin

Less than 1 EU/μg of rHuUBE2L3, His as determined by LAL method.

Molecular Weight

Approximately 18.9 kDa, a single non-glycosylated polypeptide chain containing 154 amino acids (a.a.) of human UBE2L3/UBCH7 and 8 a.a. vector sequence including 6 × His tag at N-terminus.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, 1 mM DTT, pH 7.5.

Alternative Names

UBE2L3, E2-F1, L-UBC, UBCH7, UbcM4, Human

Storage

-20C

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Research Area

Others