Recombinant Mouse Fibroblast Growth Factor-10

Cat No.

RP00457

Product Specifications

Product Type

Recombinant protein

Applications

Bioactivity, Endotoxin

Source

E. coli.

UniProt No.O35565
Purity

> 95 % by SDS-PAGE and HPLC analyses.

Bioactivity

Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 106 IU/mg.

Endotoxin

Less than 1 EU/μg of rMuKGF-2/FGF-10 as determined by LAL method.

Molecular Weight

Approximately19.5 kDa, a single non-glycosylated polypeptide chain containing 173 amino acids.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, 600 mM NaCl, pH 7.4, 1 mM mercaptoethanol.

Alternative Names

FGF-10/KGF-2, FGF10, AEY1, AEY17, BB213776, Fgf-10, Fgf5a, Gsfaey, Gsfaey17, Mouse

Storage

-20C

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Research Area

Immunology