Recombinant Mouse Insulin-like Growth Factor-1
RP00406
Product Specifications
| Product Type | Recombinant protein |
|---|---|
| Applications | Bioactivity, Endotoxin |
| Source | E. coli. |
| UniProt No. | P05017 |
| Purity | > 97 % by SDS-PAGE and HPLC analyses. |
| Bioactivity | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using serum free human MCF-7 cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 × 105 IU/mg. |
| Endotoxin | Less than 0.1 EU/μg of rMuIGF-1 as determined by LAL method. |
| Molecular Weight | Approximately 7.7 kDa, a single non-glycosylated polypeptide chain containing 70 amino acids. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH7.4. |
| Alternative Names | IGF-1, C730016P09Rik, Igf, Igf-, Igf1, Igf-I, Mouse |
| Storage | -20C |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 100mM HAc to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Research Area | Others |