Recombinant Mouse Interleukin-21
RP00368
Product Specifications
| Product Type | Recombinant protein |
|---|---|
| Applications | Bioactivity, Endotoxin |
| Source | E. coli. |
| UniProt No. | Q9ES17;E9PX58;Q5SUE2 |
| Purity | > 98 % by SDS-PAGE and HPLC analyses. |
| Bioactivity | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human N1186 T cells is less than 25 ng/ml, corresponding to a specific activity of > 4.0 × 104 IU/mg. |
| Endotoxin | Less than 0.1 EU/μg of rMuIL-21 as determined by LAL method. |
| Molecular Weight | Approximately 15.0 kDa, a single non-glycosylated polypeptide chain containing 129 amino acids. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH 7.4. |
| Alternative Names | IL-21, IL21, Mouse |
| Storage | -20C |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 C. Further dilutions should be made in appropriate buffered solutions. |
| Research Area | Immunology |