Recombinant Mouse Interleukin-3
RP00363
Product Specifications
| Product Type | Recombinant protein |
|---|---|
| Applications | Bioactivity, Endotoxin |
| Source | E. coli. |
| UniProt No. | P01586 |
| Purity | > 98 % by SDS-PAGE and HPLC analyses. |
| Bioactivity | Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of the proliferation of Mouse M-NFS-60 cells is less than 0.05 ng/ml, corresponding to a specific activity of > 2 × 107 IU/mg. |
| Endotoxin | Less than 1 EU/μg of rMuIL-3 as determined by LAL method. |
| Molecular Weight | Approximately 14.8 kDa globular protein containing 134 amino acid residues. |
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4. |
| Alternative Names | IL-3, BPA, Csfm, Csfmu, HCGF, Il, Il3, MCGF, PSF, Mouse |
| Storage | -20C |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Research Area | Immunology |