Recombinant Mouse Noggin
RP00284
Product Specifications
| Product Type | Recombinant protein |
|---|---|
| Applications | Bioactivity, Endotoxin |
| Source | E. coli. |
| UniProt No. | P97466;A2RTJ4 |
| Purity | > 95 % by SDS-PAGE and HPLC analyses. |
| Bioactivity | Fully biologically active when compared to standard. The ED50 as determined by inhibiting BMP-4-induced alkaline phosphatase production of Mouse ATDC5 cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 × 105 IU/mg in the presence of 5 ng/ml BMP-4. |
| Endotoxin | Less than 1 EU/μg of rMuNoggin as determined by LAL method. |
| Molecular Weight | Approximately 46.4 kDa, a disulfide-linked homodimer consisting of two 206 amino acid polypeptide chains. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 30 % acetonitrile, 0.1 % TFA. |
| Alternative Names | Nog, Noggin, Mouse |
| Storage | -20C |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 10 mM HAc to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Research Area | Immunology |