Recombinant Porcine Interleukin-1 Receptor Antagonist Protein
RP00154
Product Specifications
| Product Type | Recombinant protein |
|---|---|
| Applications | Bioactivity, Endotoxin |
| Source | E. coli. |
| UniProt No. | Q6PUJ3 |
| Purity | > 97 % by SDS-PAGE and HPLC analyses. |
| Bioactivity | Fully biologically active when compared to standard. The ED50 as determined by inhibiting IL-1α-dependent proliferation of Mouse D10S cells is less than 50 ng/ml, corresponding to a specific activity of > 2.0 × 104 IU/mg in the presence of 75 pg/ml rPoIL-1α. |
| Endotoxin | Less than 0.1 EU/μg of rPoIL-1RA as determined by LAL method. |
| Molecular Weight | Approximately 17.1 kDa, a single non-glycosylated polypeptide chain containing 152 amino acids. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 1 × PBS, pH 7.4, 1 mM DTT. |
| Alternative Names | IL-1RA, IRAP1, IL1RN, Porcine |
| Storage | -20C |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Research Area | Immunology |