Recombinant Rat Macrophage Inflammatory Protein-1 alpha/CCL3

Cat No.

RP00087

Product Specifications

Product Type

Recombinant protein

Applications

Bioactivity, Endotoxin

Source

E. coli.

UniProt No.P50229
Purity

> 98 % by SDS-PAGE and HPLC analyses.

Bioactivity

Fully biologically active when compared to standard. The biological activity determined by a chemoattract bioassay using human blood monocytes is in a concentration range of 10-100 ng/ml.

Endotoxin

Less than 0.1 EU/μg of rRtMIP-1α/CCL3 as determined by LAL method.

Molecular Weight

Approximately 7.9 kDa, a single non-glycosylated polypeptide chain containing 69 amino acids.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in 30 % Acetonitrile and 0.1 % TFA.

Alternative Names

MIP-1α/CCL3, MIP-1a, Scya3Rat

Storage

-20C

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Research Area

Others