Recombinant Rat Macrophage Inflammatory Protein-1 beta/CCL4

Cat No.

RP00086

Product Specifications

Product Type

Recombinant protein

Applications

Bioactivity, Endotoxin

Source

E. coli.

UniProt No.P50230
Purity

> 95 % by SDS-PAGE and HPLC analyses.

Bioactivity

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 10-1000 ng/ml.

Endotoxin

Less than 1 EU/μg of rRtMIP-1β/CCL4 as determined by LAL method.

Molecular Weight

Approximately 7.8 kDa, a single non-glycosylated polypeptide chain containing 69 amino acids.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH 7.4, 3 % trehalose.

Alternative Names

MIP-1β/CCL4, Mip1-b, Scya4, Rat

Storage

-20C

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Research Area

Immunology