Recombinant Rhesus Macaque Granulocyte Colony Stimulating Factor

Cat No.

RP00045

Product Specifications

Product Type

Recombinant protein

Applications

Bioactivity, Endotoxin

Source

E. coli.

UniProt No.A0A0D9S358
Purity

> 98 % by SDS-PAGE and HPLC analyses.

Bioactivity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using Mouse NFS-60 cells is less than 0.05 ng/ml, corresponding to a specific activity of > 2.0 × 107 IU/mg.

Endotoxin

Less than 0.1 EU/μg of rRhG-CSF as determined by LAL method.

Molecular Weight

Approximately 18.9 kDa, a single non-glycosylated polypeptide chain containing 174 amino acids.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

Alternative Names

G-CSF, CSF3, Monkey

Storage

-20C

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Research Area

Immunology