Recombinant Rhesus macaque Interleukin 1 beta

Cat No.

RP00040

Product Specifications

Product Type

Recombinant protein

Applications

Bioactivity, Endotoxin

Source

E. coli.

UniProt No.P79182
Purity

> 98 % by SDS-PAGE and HPLC analyses.

Bioactivity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using Mouse D10.G4.1 cells is less than 10 pg/ml, corresponding to a specific activity of > 1.0 × 108 IU/mg.

Endotoxin

Less than 1 EU/μg of rRhIL-1β as determined by LAL method.

Molecular Weight

Approximately 17.3 kDa, a single non-glycosylated polypeptide chain containing 153 amino acids.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

Alternative Names

IL-1β, IL-1beta, IL1B, Monkey

Storage

-20C

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Research Area

Immunology